Genetic analysis of the yeast cytoskeleton.

INTRODUCTION ..................................................................................... 259 THE CYTOSKELETON AND CELL CYCLE OF SACCHAROMYCES CEREVISIAE. 261 Mitotic Cell Cycle ............................................................... " ................. 261 Conjugation ........................................................................................ 264 Structural Genes Specifying Filamentous Cytoskeletal Components .................... 264 GENERATING MUTANTS DEFECTIVE IN THE FUNCTIONS OF KNOWN PROTEINS (PLAN B) .................................................................. 264 Isolation of Genes For Known Proteins by Homology .................................... 264 Isolation of Genes via Yeast Cytoskeletal Proteins ........................................ 266 Constructing Null Mutations .................................................................... 267 Constructing Conditional-Lethal Mutants .................................................... 268 IDENTIFICATION OF GENES BY MUTANT PHENOTYPE (PLAN A) ............... 270 Identification of Mutations "lhat Affect Cytoskeletal Function ........................... 270 Identification of Genes Whose Products Interact ........................................... 273 PHENOTYPES OF CYTOSKELETAL MUTATIONS ........................................ 277 Tubulin Mutants ................................................................................... 277 Nontubulin Mutants That Affect Spindle Function .......................................... 278 Actin Mutants ...................................................................................... 280 Ten-Nanometer Filament Mutants ............................................................. 280 CONCLUSIONS ....................................................................................... 280